Methods and use of motoneuronotrophic factor

ABSTRACT

The invention is directed to a method of administering motoneuronotrophic factors for promoting the survival, growth, proliferation, or maintenance of neurons in a severed spinal cord. The method is useful for promoting axonal regeneration and for minimizing or inhibiting the effects of scar tissue formation or other elements that prevent nerve growth.

CROSS-REFERENCE TO RELATED APPLICATIONS

[0001] This application is a divisional application directed solely tosubject matter described and claimed in the original application of U.S.patent application Ser. No. 09/989,481, filed Nov. 20, 2001, by RaymondMing Wah Chau, entitled “Methods and Use of Motoneuronotrophic Factors”,which is a continuation of U.S. patent application Ser. No. 09/592,018,filed Jun. 12, 2000, by Raymond Ming Wah Chau, entitled “Isolation andUse of Motoneuronotrophic Factors;” which is a continuation of U.S.patent application Ser. No. 08/928,862, filed Sep. 12, 1997, by RaymondMing Wah Chau, entitled “Isolation and Use of MotoneuronotrophicFactors;” which is a continuation-in-part of U.S. patent applicationSer. No. 08/751,225, filed Nov. 15, 1996, by Raymond Ming Wah Chau,entitled “Motoneuronotrophic Factors,” now abandoned; which is acontinuation in part of U.S. provisional patent application 60/026,792,by Raymond Ming Wah Chau, filed on Sep. 27, 1996, now abandoned; whichare hereby incorporated by reference in their entirety. MNTF is theabbreviation for Motoneuronotrophic Factor.

FIELD OF INVENTION

[0002] The present invention relates to the human genes which encode aspecialized group of proteins which promote the growth, maintenance,survival, and functional capabilities of selected populations ofneurons.

BACKGROUND OF INVENTION

[0003] Neuronotrophic factors (NTFs) are a specialized group of proteinswhich function to promote the survival, growth, maintenance, andfunctional capabilities of selected populations of neurons. Recentstudies have demonstrated that neuronal death occurs in the nervoussystems of vertebrates during certain periods of growth and development.However, the addition of soluble neuronal trophic factors fromassociated target tissues serves to mitigate this phenomenon of neuronaldeath. The following citations discuss neuronal trophic factors andtheir disclosures are hereby incorporated by reference: Chau, R. M. W.,et al., Neuronotrophic Factor, 6 Chin. J. Neuroanatomy 129 (1990); Kuno,M., Target Dependence of Motoneuronal Survival: The Current Status, 9Neurosci. Res. 155 (1990); Bard, Y. A., Trophic Factors and NeuronalSurvival, 2 Neuron 1525 (1989); Oppenheim, R. W., The NeurotrophicTheory and Naturally Occurring Motoneuron Death, 12 TINS 252 (1989);Bard, Y. A., What, If Anything, is a Neurotrophic Factor?, 11 TINS 343(1988); and Thoenen, H., and Edgar, D., Neurotrophic Factors, 229Science 238 (1985).

[0004] In the vertebrate neuromuscular system, the survival of embryonicmotoneurons have been found to be dependent upon specific trophicsubstances derived from the associated developing skeletal muscles.Skeletal muscles have been shown, by both in vivo and in vitro studies,to produce substances which are capable of enhancing the survival anddevelopment of motoneurons by preventing the embryonic motoneurons fromdegeneration and subsequent, natural cellular death. See O'Brian, R. J.and Fischbach, G. D., Isolation of Embryonic Chick Motoneurons and TheirSurvival In Vitro, 6 J. Neurosci. 3265 (1986); Hollyday, M. andHamburger, V., Reduction of the Naturally Occurring Motor Neuron Loss byEnlargement of the Periphery, 170 J. Comp. Neurol. 311 (1976), whosedisclosures are incorporated herein by reference. Similarly, severalinvestigators have reported that chick and rat skeletal muscles possesscertain trophic factors which can prevent the natural cellular death ofembryonic motoneurons both in vivo and in vitro. See McManaman, J. L.,et al., Purification of a Skeletal Muscle Polypeptide Which StimulatesCholine Acetyltransferase Activity in Cultured Spinal Cord Neurons, 263J.Biol. Chem. 5890 (1988); Oppenheim, R. W., et al., Reduction ofNaturally Occurring Motoneuron Death In Vitro by a Target DerivedNeurotrophic Factor, 240 Science 919 (1988); and Smith, R. G., et al.,Selective Effects of Skeletal Muscle Extract Fractions on MotoneuronsDevelopment In Vivo, 6 J. Neurosci. 439 (1986), whose disclosures areincorporated herein by reference.

[0005] In addition, a polypeptide has been isolated from rat skeletalmuscle which has been found to selectively enhance the survival ofembryonic chick motoneurons in vivo, as well the activity of cholineacetyltransferase in these motoneurons. This polypeptide has been namedCholine Acetyltransferase Development Factor (CDF) and its biologicalfunction has been demonstrated to be different from other trophicfactors such as Nerve Growth Factor (NGF), Ciliary Ganglion NeurotrophicFactor (CNTF), Brain-Derived Neurotrophic Factor (BDNF), and RetinalGanglion Neurotrophic Factor (RGNTF). See Levi-Montalcini, R.,“Developmental Neurobiology and the Natural History of Nerve GrowthFactor,” 5 Ann. Rev. Neurosci. 341 (1982); Varon, S., et al., GrowthFactors. In: Advances in Neurology, Vol. 47: Functional Recovery inNeurological Disease, Waxman, S. G. (ed.), Raven Press, New York, pp.493-521 (1988); Barde, Y. A., Trophic Factors and Neuronal Survival, 2Neuron 1525 (1989); Chau, R. M. W., et al., The Effect of a 30 kDProtein from Tectal Extract of Rat on Cultured Retinal Neurons, 34Science in China, Series B, 908 (1991), whose disclosures areincorporated herein by reference.

[0006] The inventor of the invention disclosed in the instantapplication, Dr. Raymond Ming Wah Chau, presented results which reportedthe isolation and characterization of two motoneuronotrophic factorsfrom rat muscle tissue having apparent molecular weights of 35 kD and 22kD. This data was initially disseminated in 1991 at the World Congressof Cellular and Molecular Biology held in Paris, France. See Chau, R. M.W., et al., Muscle Neuronotrophic Factors Specific for Anterior HornMotoneurons of Rat Spinal Cord. In: Recent Advances in Cellular andMolecular Biology, Vol. 5, Peeters Press, Leuven, Belgium, pp. 89-94(1992), the disclosure of which is hereby incorporated by reference.This 35 kD protein has been defined by Dr. Chau as motoneuronotrophicfactor 1(MNTF1) and the apparent 22 kD protein as motoneuronotrophicfactor 2 (MNTF2). These two trophic factors have been demonstrated invitro by Dr. Chau to support the growth and/or regeneration of bothisolated anterior horn motoneurons and spinal explants of rat lumberspinal cord.

[0007] Subsequently, in 1993, Dr. Chau reported the successful cloningof human MNTF1, a protein having an apparent molecular weight of 55 kD,and its associated receptor from a human retinoblastoma cDNA library.See Chau, R. M. W., et al., Cloning of Genes for Muscle-DerivedMotoneuronotrophic Factor 1 (MNTF 1) and Its Receptor by MonoclonalAntibody Probes, (abstract) 19 Soc. for Neurosci. part 1, 252 (1993),the disclosure of which is hereby incorporated by reference. The clonedhuman MNTF1 was demonstrated to have biological activity similar to thatof the “native” MNTF1 protein in that it supported the in vitro growthof rat anterior horn motoneurons.

[0008] Although various biological aspects of MNTF1 have been widelypublicized in scientific journals, the DNA and inferred amino acidsequences of the cloned human MNTF1 gene and its associated receptor hadnot yet been made publicly available by Dr. Chau, nor had thesesequences been confirmed by peer-review within the field. Moreover, thecloned human MNTF1, reported by Dr. Chau in 1993, was not in a formwhich was amenable to being sub-cloned into an appropriate vector, suchas an in vitro mammalian expression system. Thus, there remained a needfor the human MNTF1 gene to be properly manipulated, sequenced,sub-cloned into an appropriate vector(s), sub-cloned into an appropriateexpression system(s) and associated host(s), as well as the isolationand purification of the resulting recombinant human MNTF1 protein forsubsequent potential utilization in human therapeutic modalities.

SUMMARY OF THE INVENTION

[0009] The present invention is directed to a family ofmotoneuronotrophic factors including MNTF1 and MNTF2, which have beenshown to have diagnostic and therapeutic applications in mammals. Thepresent invention is also directed to novel DNA sequences which encodemotoneuronotrophic factors, including recombinant human MNTF1-F3 (SEQ IDNO:1) and MNTF1-F6 (SEQ ID NO:2, and SEQ ID NO:5), to vectors whichcontain these novel DNA sequences, to expression systems and associatedhosts which contain these novel DNA sequences, and the novel recombinanthuman MNTF1-F3 (SEQ ID NO:3) and MNTF1-F6 (SEQ ID NO:4) proteins whichare produced by the aforementioned expression systems.

[0010] The invention further includes a method for promoting thesurvival, growth, proliferation, or maintenance of mammalian neuronscomprising administering to the neurons an effective amount of apurified polypeptide comprising an amino acid sequence that is at least80% identical to SEQ ID NO:3 or SEQ ID NO:4.

[0011] formation. In specific embodiments, the method comprisespromoting regeneration of the axon of a motoneuron; promoting thesurvival, growth, proliferation, or maintenance of neurons in vitro;promoting the survival, growth, proliferation, or maintenance ofisolated spinal motoneurons in vitro; administering the polypeptide tonon-neuronal cells or tissues sufficiently proximal to neurons such thatthe polypeptide is effective at promoting the survival, growth,proliferation, or maintenance of mammalian neurons; promoting the rescueand morphologically-complete neuronal regeneration of axotomizedmotoneurons in vivo; promoting the regeneration of nerve fibers in asevered or injured spinal cord of a mammal; promoting the regenerationof peripheral nerves in a mammal; promoting the axonal regeneration ofaxotomized motoneurons in a mammal; inhibiting the effects of hereditarymotoneuron disease in a mammal where muscles associated with thediseased motoneurons degenerate; administering the polypeptide in asuitable carrier to a mammal for treatment of a medical conditionselected from the group consisting of: peripheral nerve injuries,musculoskeletal disorders, spinal cord injuries, head injuries, strokes,neuromuscular degenerative diseases, amyotrophic lateral sclerosis,spinal muscular atrophy, peripheral neuropathy, inhibition of scarformation, diabetic peripheral neuropathy, peripheral neuropathyresulting from AIDS, peripheral neuropathy resulting from radiationtreatment for cancer, multiple sclerosis, muscular dystrophy, myastheniagravis, and sensory neuronal function disorders.

[0012] In other embodiments, the invention includes a method ofpromoting the differentiation of neural stem cells into neural cellscomprising administering to the neural stem cells an effective amount ofa purified polypeptide comprising an amino acid sequence that is atleast 80% identical to SEQ ID NO:4 or SEQ ID NO:3. In one embodiment,the method comprises administering the polypeptide to non-neuronal cellsor tissues sufficiently proximal to neural stem cells or tissuessufficiently proximal to neural stem cells such that the polypeptide iseffective at promoting the differentiation of the neural stem cells intoneurons.

DESCRIPTION OF THE FIGURES

[0013] The present invention may be better understood and its advantagesappreciated by those individuals skilled in the relevant art byreferring to the accompanying figures wherein:

[0014]FIG. 1A depicts the DNA sequence of the MNTF1-1443 DNA fragment(SEQ ID NO:1) which encodes the MNTF1-F3 protein. By standardconvention, the DNA sequence is shown in the 5′ to 3′ directed.

[0015]FIG. 1B depicts the DNA sequence of the MNTF1-927 DNA fragment(SEQ ID NO:2) which encodes the MNTF1-F6 protein. The first 99 of thesenucleotides (SEQ ID NO:5) correspond to and code for the 33 amino acidpeptide shown in FIG. 2B. By standard convention, the DNA sequence isshown in a 5′ to 3′ orientation.

[0016]FIG. 1C depicts the first 99 nucleotides (SEQ ID NO:5) (33 codons)of the DNA sequence shown in FIG. 1B.

[0017]FIG. 2A depicts the direct amino acid sequence of MNTF1-F3 protein(SEQ ID NO:3). By standard convention, the amino acid sequence isreported from the amino (NH2—) terminus to the carboxyl (—COOH)terminus.

[0018]FIG. 2B depicts the direct amino acid sequence of MNTF1-F6 protein(SEQ ID NO:4) encoded by the MNTF1-927 DNA fragment. By standardconvention, the amino acid sequence is reported from the amino (NH2—)terminus to the carboxyl (—COOH) terminus.

[0019]FIGS. 3A-3C depict the “Protein Band-Fishing By Cells” methodologywhich showed that isolated anterior horn motoneurons survived and grewon PhastGel regions containing MNTF1 and MNTF2 from muscle extract whichhad been electrophoretically separated into protein bands within thePhastGel. FIGS. 3A-3C illustrate the results obtained utilizing the“Protein Band-Fishing By Cells” methodology to isolate themotoneuronotrophic factors MNTF1 and MNTF2.

[0020]FIG. 4 (panels 1-4) reveals the results of isolated motoneuronscultured with the 35 kD gel band containing the MNTF1 for 3, 7, and 14days (panels 2, 3, & 4, respectively) and a control culture (panel 1)without the 35 kD MNTF1-containing gel.

[0021]FIG. 5 (panels 1-6) reveals the immunohistochemical distributionof MNTF1 (panels 1, 3 & 5) and its putative MNTF1 receptor (panels 2, 4& 6) in muscle (panels 1 & 2); Schwann cells (panels 3 & 4); andmotoneurons (panels 5 & 6).

[0022]FIG. 6 depicts the axotomy of the sciatic nerve of a 10 day-oldSprague Dawley rat. The sciatic nerve at the gluteal region of the rightside was axotomized with 1 mm of the nerve removed and MNTFs weresubsequently placed around the cut edge of the nerve.

[0023]FIGS. 7A and 7B reveal the synergistic effect of MNTF1(35 kD) andMNTF2 (22 kD) on the survival of axotomized motoneurons of the sciaticnerve in the Sprague Dawley rats. Panel 1 had no gel added (NG) andpanel 3 had 22 kD MNTF2 gel added; (I) is the intact side and (A) is theaxotomized side. Panel 2 had the 35 kD MNTF1 gel added and panel 4 hadboth the 35 kD and 22 kD gels added.

[0024]FIGS. 8A and 8B provide histogram and tabular results of thesynergistic protection and rescue of axotomized motoneurons of thesciatic nerve by the partially-purified and isolated motoneuronotrophicfactors MNTF1 and MNTF2 following the transection of the sciatic nervein Sprague Dawley rats.

[0025]FIG. 9 (panels 1-4) reveals the microscopic effects of MNTFs onthe survival of motoneurons in the facial nuclei of the transectedfacial nerve in the Sprague Dawley rats. Panel (1) the facial nuclei ofthe normal intact control rat after two weeks; panel (2) the axotomizedfacial nuclei without factors after two weeks; panels (3) and (4) theaxotomized facial nuclei with 35 kD MNTF1 and with both 35 kD and 22 kDMNTF1 & MNTF2, respectively, for two weeks.

[0026]FIGS. 10A and 10B provide histogram and tabular results of the invivo effects of muscle-derived motoneuronotrophic factors (22 kD, 35 kD,and 22 kD+35 kD) on neuronal survival following the transection of thefacial nerve in Sprague Dawley rats.

[0027]FIGS. 11A and 11B provide histogram and tabular results of the invivo effects of muscle-derived 35 kD MNTF1 motoneuronotrophic factor onneuronal survival following the transection of the facial nerve inSprague Dawley rats in the presence of goat anti-rabbit IgG or anti-35kD motoneuronotrophic factor monoclonal antibody.

[0028]FIG. 12A-D depicts a series of four photomicrographs demonstratingthe in vivo effects of the 35 kD MNTF1 and 22 kD MNTF2 proteins uponneuronal survival following the transection of dorsal root ganglion inSprague Dawley rats.

[0029]FIG. 13 shows a hemisection of the spinal cord at the T1-T3 levelin the adult Sprague Dawley rat depicting the surgical autographing ofthe ulnar nerve to the ventral spinal cord and the median nerve to thedorsal spinal cord.

[0030]FIG. 14 (panels 1-6) depicts the trophic and rescuing effects ofMNTFs on the survival of motoneurons following the hemisection of thespinal cord in Sprague Dawley rat with nerve autographs. Panels 1-4demonstrate that: (1) with the presence of MNTF1 & MNTF2 there werereduced in inflammation, scar formation, and white blood cellsinfiltration at the junction of the autograph nerve (N) and the spinalcord (C); panel (2) with no MNTFs there were increased in scarformation, inflammation, and WBCs infiltration at the junction of theautograph nerve (N) and the spinal cord (C); panel (3) is an electronmicrographs of the autograph nerve with MNTFs which reveals an increasein number and size of the regenerating nerve fibers with healthymyelination; panel (4) is an EM of the autograph nerve without MNTFswhich reveals no healthy myelinated regenerating nerve fibers.

[0031]FIGS. 15A and 15B depict the body weights of homozygous (N1-N4) inpanel A and heterozygous (N1-N4) in Panel B wild type mice verses thebody weights of their litter-mates wobbler mice which were treated withrat MNTFs (W5-W8) and (W1-W4), respectively.

[0032]FIG. 16 (panels A-F) depicts the survival (with neurite outgrowth)of in vitro isolated anterior motoneuron cultures derived from thelumbar spinal cord following 14 days of co-culture with humanrecombinant MNTF1 (hrMNTF1). Panels a-c isolated motoneuron cultureswere photographed at day 2 and panels d-e photographed at day 14; panelsa and d represent control cultures with no hrMNTF1; and panels b, c, e,and f depict experimental cultures with hrMNTF1.

[0033]FIG. 17 (panels 1-6) depicts the survival of in vitro-isolatedanterior motoneuron cultures derived from the lumbar spinal cordfollowing 8 days of co-culturing without MNTF1-F6 (panel 1) and MNTF1-F3(panel 2); with GST-fusion protein of MNTF1-F6 (panel 3) and MNTF1-F3(panel 4); and with cleaved protein of MNTF1-F6 (panel 5) and MNTF1-F3(panel 6). Low magnification (×100).

[0034]FIG. 18 (panels 1-6) depicts the survival (with neurite outgrowth)of in vitro-isolated anterior motoneuron cultures as in FIG. 17 (1-6)above. High magnification (×320).

[0035]FIG. 19 (panels 1-2) depicts the survival with neurite outgrowthof in vitro-isolated anterior motoneuron cultures following 8 days ofco-culturing with the cleaved protein of MNTF1-F6. High Magnification(×400).

[0036] FIGS. 20A-D depicts the survival, growth and regeneration ofmyelinated nerve fiber from the in vitro-isolated anterior motoneuronsfrom the culture as originally demonstrated in FIGS. 17 (panel 5), 18(panel 5), and 19 (panels 1-2) after 21 days of co-culturing with thecleaved protein of MNTF1-F6. FIG. 20A reveals nerve fibers; one 1.5 mmand the other 3 mm were regenerated from the cultured motoneurons. Lowmagnification (×40). FIG. 20B is a high magnification (×140) of FIG. 20Aand a composite picture to reveal the myelinated, regenerated nervefiber with oligodendrocytes (possible Schwann cells?*) attached. FIG.20C and FIG. 20D reveal clearly the axon cylinders in the innerregenerated myelinated nerve fiber with node of Rannier. Highmagnification (×400).

DESCRIPTION OF THE INVENTION

[0037] The present invention comprises a family of neuronotrophicfactors which possess the ability to exert a trophic effect onmotoneurons and the genes which encode these factors. These factors havebeen isolated and the genes which encode these factors have been clonedand expressed, and both the nucleic acid and polypeptide sequencesprovided

[0038] It has been demonstrated that the isolated factors, theexpressed, recombinant factors, and chemically synthesized polypeptidefactors are capable of inducing the continued viability and neuriteoutgrowth of motoneurons. Therefore, these factors have been classifiedas “motoneuronotrophic factors” or “MNTFs.”

[0039] MNTFs have been isolated from both rat and human sources andtheir biological activities have been examined both in vivo and invitro. For example, their potential biological activity has beenexamined in both surgically-axotomized and hereditarily diseasedanimals.

[0040] The MNTFs disclosed in the present invention are useful for avariety of purposes, including: (1) for promoting axonal regeneration;(2) for promoting the survival, growth, proliferation, differentiationor maintenance of mammalian neurons; (3) for treatment of (preventionand /or reduction of the severity) of neurological conditions derivingfrom a) acute, subacute, or chronic injury to the nervous system,including traumatic injury, chemical injury, vassal injury and deficits(such as the ischemia resulting from stroke), together withinfectious/inflammatory and tumor-induced injury, b) aging of thenervous system, c) chronic immunological diseases of the nervous systemeor affecting the nervous system, including multiple sclerosis, d)chronic neurodegenerative diseases of the nervous system includinghereditary motoneuron diseases such as amyotrophic lateral sclerosis,spinal muscular atrophy; (4) for peripheral nerve injuries,musculoskeletal disorders, spinal cord injuries, head injuries,peripheral neuropathy, diabetic peripheral neuropathy, peripheralneuropathy resulting from AIDS, peripheral neuropathy resulting fromradiation treatment for cancer, muscular dystrophy, myasthenia gravis,and sensory neuronal function disorders; (5) for promoting or regulatingdifferentiation of neural stem cells into neural cells; (6) forminimizing or inhibiting the effects of scar tissue formation; and (7)for accelerating wound healing while concomitantly minimizing orinhibiting the effects of scar tissue and keloid formation.

[0041] MNTF can be utilized in the diagnosis of motoneuron diseases byexploiting their effectiveness in altering the symptomology of thedisease. For example, a detailed description is provided below whichsupplies evidence of the successful use of MNTF1-F3 and MNTF1-F6 inwobbler mice. Wobbler mice are animals which possess double recessivegenes for a hereditary motoneuron disease. The motoneuron diseasemanifests itself with symptoms of upper limb neuromuscular failure whichinitially appears approximately 3 weeks after birth. The condition alsoaffects the animal's body weight, general health conditions,respiration, and life span in a deleterious manner. The diseasegradually progresses to the final, terminal stage (defined as stage 4)by 3 months of age, with an associated dramatic increase in animalmortality. The symptomatic responsiveness of the wobbler mice to theaforementioned MNTFs is indicative of the defect occurring at themotoneuron level, thus serving to confirm various genetic evidence whichhas established the hereditary nature of the defect.

[0042] In addition, MNTFs may also be utilized therapeutically to treatdamaged or diseased motoneurons. For example, a detailed description isprovided below which includes experiments using these factors onsurgically-transected motoneurons, with the resulting recovery of alarge percentage of the transected motoneurons. Also described is theutilization of the factors in the aforementioned hereditary motoneurondisease, the wobbler mouse, with results demonstrating that the treatedanimals survived, with a concomitant arresting of pathologicalsymptomologies, and thrived for much longer periods of time than thoseof the untreated animals.

“Protein Band-Fishing by Cells” Methodology

[0043] Rat MNTF1 and MNTF2 were isolated utilizing the “ProteinBand-Fishing by Cells” methodology as reported in Chau, R. M. W., etal., Muscle Neuronotrophic Factors Specific for Anterior HornMotoneurons of Rat Spinal Cord. In: Recent Advances in Cellular andMolecular Biology, Vol. 5, Peeters Press, Leuven, Belgium, pp. 89-94(1992). Trophic factors are generally found in minute quantities invivo; hence this can potentially cause tremendous difficulties in theirisolation utilizing traditional biochemical methodologies. In view ofthis fact, a novel technique designated “Protein Band-Fishing by Cells”was developed in which viable anterior horn motoneurons were co-culturedon an electrophoretic gel containing the separated proteins from ratperoneal muscle. This methodology thus allowed the viable anterior hornmotoneurons to “fish-out” those peroneal muscle proteins which exhibitedbiological activity specific for those motoneurons (i.e., by thedemonstration of continued viability and growth in vitro).

[0044] For electrophoretic protein separation, the peroneal longus andbrevis skeletal muscles, without the associated tendon, from 3 week-oldSprague Dawley rats were aseptically dissected into small sections andwashed 3-times in Ca⁺²/Mg⁺²-free Hank's medium. The muscle tissue wasthen homogenized in 10 mM Tris-HCl (pH 7.2). A cell lysate was obtainedby centrifugation at 100,000×g. for 10 minutes, and the supernatant wasfiltered utilizing a 0.2 μm Millipore filter membrane (Millipore Corp.,Mass.). The resultant filtrate was designated muscle extract (Me).

[0045] For cell culture, the spinal cords from four 3 week-old rats wereexcised and the meninges and any associated vessels were removed. Itshould be noted that the motoneurons were pre-labeled with rhodamine Bfor subsequent microscopic visualization by implantation of gelfoamscontaining rhodamine B into the peroneal muscles of the experimentalanimals one week prior to their sacrifice. The gray matter of theanterior horn region of segments L4-L5 was subsequently removed fromeach excised spinal cord, washed several times in Ca⁺²/Mg⁺²-freemediumn, and treated with collagenase (0.08%) at 37EC for 60 minuets.Following gentle dissociation and sedimentation, the cell were suspendedin RPMI 1640 medium/6% fetal calf serum/20 mM HEPES/1× streptomycin andpenicillin and utilized for subsequent PhastGel culture.

[0046] The filtered, muscle extract (Me, 10-20 1 μl of a 1 mg/mlsolution) was then applied to a pre-cast 20% native PhastGel (50×40×0.45mm, Pharmacia LKB Biotech AB, Upsala, Sweden) for separation by thePhast System gel electrophoresis (Pharmacia LKB Biotech AB, Upsala,Sweden). The electrophoretic conditions utilized were those suggested bythe computer program of Olsson, I., et al., Computer Program forOptimizing Electrophoretic Protein Separation, 9 Electrophoresis 16(1988).

[0047] Following electrophoresis, the middle portion of the PhastGel wasremoved via aseptic technique for subsequent culture with ˜1×10⁶ spinalcord cells/gel. The remaining portions of the PhastGel were stainedusing the silver stain method to identify and locate the resultingprotein bands. Following 2-7 days of incubation, the PhastGel/spinalcell culture was fixed in 0.4% paraformaldehyde in Phosphate-bufferedsaline (PBS) for approximately 2 hours. This portion of the gel was thenreinserted back into its original position of the PhastGel and examinedfor viable, large (˜25 mm) rhodamine B retrograde-prelabeled motoneuronsin the cultured spinal cells using either an inverted phase (ZeissAxiophot Inverted Microscope, West Germany) or a fluorescent microscope(Zeiss, West Germany).

[0048] Silver staining demonstrated a total of 34 native protein bands(26 protein bands with an apparent molecular weight of >30 kD and 8protein bands with an apparent molecular weight of <30 kD) separatedfrom the muscle extract. These results are illustrated in FIGS. 3A-3C.Interestingly, large (˜25 mm) rhodamine B retrograde-prelabeledmotoneurons from the cultured spinal cells were found to survive in onlytwo spatially-distinct regions corresponding to the 35 kD and 22 kDapparent molecular weight protein bands. These results are demonstratedin FIG. 3B. Moreover, silver staining methodology demonstrated heavierstaining of the 35 kD protein band, in comparison to the 22 kD proteinband, thus potentially reflecting the relative concentrations of thesetwo trophic factors in the muscle extract. No other types of neuronalcells were found to have survived, nor did the aforementionedmotoneurons remain viable in any other locations corresponding toadditional proteins with differing apparent molecular weights. Theseaforementioned results are indicative of the large, rhodamine Bretrograde-prelabeled motoneurons having “fished out,” from the amongstthe 34 total peroneal muscle protein bands, their own trophic factorshaving apparent molecular weights of 35 kD and 22 kD, and areillustrated in FIG. 3C. The 35 kD and 22 kD protein bands weredesignated MNTF1 and MNTF2, respectively.

Determination of MNTF1 and MNTF2 Biological Activity

[0049] As used herein, the terms “biologically active polypeptide” and“biologically active fragment” refer to a peptide or polypeptide inaccordance with the above description for MNTF factors wherein the MNTFacts as a growth factor for neurons or neural stem cells.

[0050] As used herein, the terms “administer” and “administer toneurons” includes applying the purified polypeptide to non-neuronalcells or tissues sufficiently proximal to neurons such that thepolypeptide is effective at promoting the survival, growth,differentiation, or maintenance of mammalian neurons or neural stemcells.

[0051] The invention encompasses biologically active mammalianpolypeptides and fragments that comprise an amino acid sequence which isat least 80% identical to the amino acid sequence of SEQ ID NO:3 andmethods of using the polypeptide to promote the survival, growth,proliferation, or maintenance of mammalian neurons and thedifferentiation of neural stem cells into neurons. Preferably thepolypeptide is at least 85% identical to the amino acid sequence of SEQID NO:3. Further preferable, the polypeptide is at least 90% identicalto the amino acid sequence of SEQ ID NO:3. Still further preferable, thepolypeptide is at least 95% identical to the amino acid sequence of SEQID NO:3, or at least 99% identical to the amino acid sequence of SEQ IDNO:3. In a particularly preferred embodiment, the polypeptide is 100%identical to the amino acid sequence of SEQ ID NO:3. Alternatively, thepolypeptide comprises the amino acid sequence of SEQ ID NO:3 with one ormore conservative amino acid substitution.

[0052] A “conservative substitution” is one in which an amino acid issubstituted for another amino acid that has similar properties, suchthat one skilled in the art of peptide chemistry would expect thesecondary structure and hydropathic nature of the polypeptide to besubstantially unchanged. A conservative amino acid substitution occurswhen one amino acid residue is replaced with another that has a similarside chain. Amino acid residues having similar side chains are known inthe art and include families with basic side chains (e.g., lysine(Lys/K), arginine (Arg/R), histidine (His/H)), acidic side chains (e.g.,aspartic acid (Asp/D), glutamic acid (Glu/E)), uncharged polar sidechains (e.g., glycine (Gly/G), asparagine (Asn/N), glutamine (Gln/Q),serine (Ser/S), threonine (Thr/T), tyrosine (Tyr/Y), cysteine (Cys/C)),nonpolar side chains (e.g., alanine (Ala/A), valine (ValIV), leucine(Leu/L), isoleucine (Ile/I), proline (Pro/P), phenylalanine (Phe/F),methionine (Met/M), tryptophan (Tip/W)), beta-branched side chains(e.g., threonine (Thr/T), valine (ValIV), isoleucine (Ile/I)) andaromatic side chains (e.g., tyrosine (Tyr/Y), phenylalanine (Phe/F),tryptophan (Trp/W), histidine (His/H)). In the case of polypeptidesequences which are less than 100% identical to a reference sequence,the non-identical positions are preferably, but not necessarily,conservative substitutions for the reference sequence.

[0053] The invention further includes biologically active mammalianpolypeptides, including for example, those that are isolated, expressedin vitro, or chemically synthesized, that comprise an amino acidsequence which is at least 80% identical to the amino acid sequence ofSEQ ID NO:4 and methods of using the polypeptide to promote thesurvival, growth, proliferation, or maintenance of mammalian neurons andthe differentiation of neural stem cells into neurons. Preferably thepolypeptide is at least 85% identical to the amino acid sequence of SEQID NO:4. Further preferable, the polypeptide is at least 90% identicalto the amino acid sequence of SEQ ID NO:4. Still further preferable, thepolypeptide is at least 95% identical to the amino acid sequence of SEQID NO:4. In a particularly preferred embodiment, the polypeptide is 100%identical to the amino acid sequence of SEQ ID NO:4. In alternativeembodiments, the invention includes polypeptides comprising at least 10consecutive amino acid residues of sequence of SEQ ID NO:4, at least 15consecutive amino acid residues of sequence of SEQ ID NO:4, at least 20consecutive amino acid residues of sequence of SEQ ID NO:4, at least 25consecutive amino acid residues of sequence of SEQ ID NO:4, and at least30 consecutive amino acid residues of sequence of SEQ ID NO:4.Alternatively, the polypeptide comprises the amino acid sequence of SEQID NO:4 with one or more conservative amino acid substitution.

[0054] The rat MNTF1 and MNTF2 proteins were then tested for potentialbiological activity with anterior horn motoneurons. Anterior hornmotoneurons were isolated from four 3 week-old Sprague Dawley rat lumbarspinal cord via collection of the associated gray matter. The collectedgray matter was then digested with a 0.08% collagenase solution in DMEMmedium. The motoneurons in the DMEM medium were collected in 5 mlaliquots and allowed to settle at room temperature for 2 minutes. Theupper 4.5 ml of supernatant was aspirated and discarded and theremaining media, containing the motoneurons, were subsequently utilizedto test the biological activity of the rat MNTF1 and MNTF2 proteins.

[0055] Rat MNTF1 and MNTF2 were isolated utilizing the aforementionedPhast System gel electrophoresis apparatus by aseptic excision of thePhastGel sections (˜1×30 mm) containing the 35 kD and 22 kD proteinbands, respectively (see FIGS. 3A-3C). Anterior horn motoneurons,isolated from the lumbar spine of 3 week-old Sprague Dawley rats, werethen co-cultured with and without the presence of MNTF1- andMNTF2-containing PhastGel sections. Results indicated that both MNTF1-and MNTF2-containing PhastGel sections were capable of supporting thecontinued viability of the anterior horn motoneurons, as well assupporting neurite outgrowth.

[0056] In a similar experiment, depicted in FIG. 4, panels 1-4, anteriorhorn motoneurons, isolated from the lumbar spine of 3 week-old SpragueDawley rats, were then co-cultured with and without the presence ofMNTF1-containing PhastGel sections for 3, 7, and 14 days (panels 2, 3, &4, respectively). The results of this experiment are shown in FIG. 4which demonstrates that MNTF1 is capable of supporting the continuedviability of anterior horn motoneurons for 2 or more weeks. Thedetermination of motoneuron viability and neurite outgrowth wasperformed utilizing both MTT micro-assay and microscopic morphologicalmeasurement. MTT is a highly sensitive, colorimetric tetrazoliumderivative

[0057] (3-(4,5-dimethylthiazol-2-yl)-2,5-dipheny tetrazolium bromide)which can be converted by viable cells to form a formazan product whichcan subsequently be measured with an ELISA colorimeter. The controlassay (panel 1), utilizing selected remaining protein-containingPhastGel sections or PhastGel sections alone, failed to support anycontinued viability or neurite outgrowth of the anterior hornmotoneurons.

In Vivo Testing of Rat MNTF1 and MNTF2 in Axotomized Animals

[0058] In vivo testing of rat MNTF1 and MNTF2 were next performedutilizing axotomized animals. Axotomized rat sciatic, facial,hypoglossal, and musculocutaneous nerves were cut in the right side of10 day-old Sprague Dawley rats. The aforementioned nerves were leftintact on the left side of the rats to serve as contralateral internalcontrols. Sections of MNTF1- and/or MNTF2-containing PhastGel (˜2×3 mmsections containing 5-30 ng of MNTFs) were applied via implantation tothe axotomized sites. The axotomy of the sciatic nerve and subsequentimplantation of MNTF-containing gels(s) are illustrated in FIG. 6. Inthe surgical procedure, the right-side of the gluteal region wasdissected so as to reveal the sciatic nerve. A 1 mm section of thesciatic nerve was resected and the remaining ends of the nerve werespatially aligned without performing an end-to-end anastomosis. TheMNTF-containing and control gel sections were then placed around thesurgical resection site and the initial incision was closed. Axotomizedanimals in which PhastGel without MNTFs were implanted in an analogousmanner and were utilized as controls. After periods of 2, 4, and 8weeks, the axotomized animals were sacrificed and tissue sections wereexcised and prepared for determination of motoneuron survival/viabilityvia microscopic examination.

[0059] The results demonstrated that without the application of theMNTFs, 60-70% of the motoneurons associated with the aforementionednerves had degenerated, therefore only 30-40% of the motoneurons werestill viable at the time of microscopic examination. Conversely, in thepresence of either MNTF1 or MNTF2, 55-75% of the motoneurons displayedcontinued viability during the above periods of observation. In thepresence of both MNTF1 and MNTF2, ˜90% of the motoneurons were found toexhibit continued viability.

[0060]FIGS. 7 and 8 depict the results of the in vivo effects ofmuscle-derived motoneuronotrophic factors on neuronal survival followingthe transection of the sciatic nerve. As previously discussed, thesciatic nerves were cut in the right side of 10 day-old Sprague Dawleyrats (see FIG. 6); whereas the sciatic nerves were left intact on theleft side of the rats to serve as contralateral internal controls.Section of MNTF1-containing and/or MNTF2-containing PhastGel (˜2×3 mmsection containing 5-30 ng of MNTFs) were applied via implantation tothe axotomized sites. MNTF1 and MNTF2 correspond to the 35 kD and 22 kDproteins, respectively. PhastGel sections without MNTFs were implantedin axotomized animals in an analogous manner, and served as controls.After 3 weeks, the axotomized animals were sacrificed and sections ofthe lumbar spinal cord were excised and prepared for determination ofmotoneuron survival/viability via microscopic examination. Resultsdemonstrated that without the application of the MNTFs, 56% of themotoneurons associated with the aforementioned nerves had degenerated.Therefore, only 44% of the motoneurons were still viable at the time ofmicroscopic examination. Conversely, in the presence of either the 35 kDprotein MNTF1 or the 22 kD protein MNTF2, 76% and 71% of the motoneuronsdisplayed continued viability, respectively, at the time of microscopicevaluation.

[0061]FIG. 7A and 7B represents a series of eight photomicrographsdepicting the in vivo effects of muscle-derived motoneuronotrophicfactor on neuronal survival following transection of the sciatic nervein Sprague Dawley rats. These results are presented in tabular andhistogram form in FIG. 8A and 8B, respectively. In this series ofexperiments, axotomized sciatic nerves were cut in the right side ofadult Sprague Dawley rats, whereas the left side sciatic nerve was leftintact as an internal contralateral control. Sections ofMNTF1-containing or MNTF2-containing PhastGel (˜2×3 mm sectioncontaining 5-30 ng of MNTFs) were applied via implantation to theaxotomized sites. PhastGel sections without MNTFs were implanted inaxotomized animals in an analogous manner, and served as controls. Itshould be noted that all the lumbar 4 and 5 anterior horn motoneuronswere retrogradely prelabeled with horseradish peroxidase (HRP) for 2weeks prior to animal sacrifice, at which time sections of the sciaticnerve were excised and prepared for subsequent determination ofmotoneuron survival/viability via a microscopic cell countingmethodology.

[0062] Results shown within the control photomicrographs (see FIG. 7A,panel 1) demonstrated that without the application of the MNTFs (NG), acomparatively low number of viable (i.e., HRP-labeled) motoneurons arepresent in all the serial sections of the axotomized side (A) of thelumbar spinal cord as compared to the intact side. This finding iscorroborated by the tabular results presented in FIG. 8 which show that56% of the HRP-labeled motoneurons associated with the sciatic nerve haddegenerated. Conversely, in the presence of the 22 kD protein MNTF2 (seeFIG. 7A, panel 3), the 35 kD protein MNTF1 (see FIG. 7B, panel 2), orboth the 22 kD and 35 kD MNTFs (see FIG. 7B, panel 4) a markedly higherrate of survival of the HRP-labeled motoneurons was demonstrated. Theseresults are quantitatively depicted in FIG. 8A and 8B, and arecorroborated by the fact that the utilization of the 35 kD MNTF1 proteinor the 22 kD MNTF2 protein resulted in 76.5% and 71.3% of theHRP-labeled motoneurons displaying continued viability, respectively.Similarly, in the presence of both MNTF1 and MNTF2 (see FIG. 7B, panel4), a large number of viable motoneurons are found with 86.8% of themotoneurons exhibiting continued viability as a result of treatment withboth MNTFs (see FIG. 8A and 8B).

[0063]FIG. 9, panels 1-4 illustrates the results of the in vivo effectsof MNTF application on neuronal survival and viability following thetransection of the rat facial nerve in photomicrographic form. In thisseries of experiments, axotomized facial nerves were cut in the rightside of 10 day-old Sprague Dawley rats, whereas the left-side facialnerves were left intact to serve as an internal contralateral control.Sections of MNTF1-containing or MNTF2-containing PhastGel (˜2×3 mmsection containing 5-30 ng of MNTFs) were applied via surgicalimplantation to the axotomized sites. As before, the MNTF1 and MNTF2correspond to the 35 kD and 22 kD proteins, respectively. PhastGelsections without MNTFs were implanted in axotomized animals in ananalogous manner, and served as controls. After periods of 1 and 2weeks, the axotomized animals were sacrificed and serial sections of thefacial nuclei in the brain were prepared for subsequent evaluation ofmotoneuron survival/viability via microscopic examination followinghistological staining. Results in FIG. 9, panel 1 depicts the facialnuclei of the normal intact control rat after two weeks; panel 2 depictsthe axotomized facial nuclei without factors after two weeks; panels 3and 4 depict the axotomized facial nuclei with 35 kD MNTF1 and with both35 kD and 22 kD MNTF1 & MNTF2, respectively, after two weeks. FIGS. 10Aand 10B illustrate the results in tabular and histogram form,respectively. The results shown in FIGS. 9, 10A and 10B demonstratedthat without the application of either the MNTFs, ˜50% of themotoneurons associated with the facial nuclei had degenerated after onlyone week and ˜65% had degenerated after two weeks (panel 1). Incontrast, in the presence of either the 35 kD protein MNTF1 (panel 3) orthe 22 kD protein MNTF2 (panel 2), ˜70% of the motoneurons displayedcontinued viability after 1 week and ˜55% were still viable after 2weeks. Similar results were obtained when both the 35 kD (MNTF1) and 22kD (MNTF2) proteins were utilized (panel 4).

[0064]FIGS. 11A and 11B depict the results of the in vivo effect ofmuscle-derived motoneuronotrophic factors on neuronal survival andviability following the transection of the rat facial nerve in thepresence of either goat anti-rabbit IgG or an anti-35 kD monoclonalantibody in tabular and histogram form, respectively. The development ofthe anti-35 kD MNTF1 monoclonal antibody will be discussed in asubsequent section herein. The goat anti-rabbit IgG was utilized as acontrol to ensure that there was no inhibition of the biologicalactivity of the 35 kD MNTF1 protein due to non-specific interactions.Results indicated that

[0065] ˜80% of the motoneurons associated with the facial nerve werestill viable after a 2 week period. In contrast, a marked reduction inmotoneuron viability (50% viability after 2 weeks) was found when ananti-35 kD MNTF1 monoclonal antibody was utilized. Therefore, thediminution of neuronal viability appeared to be a function of theinhibition of the 35 kD MNTF1 protein by the anti-35 kD MNTF1 monoclonalantibody.

[0066] FIGS. 12A-D are a series of four photomicrographs depicting thein vivo effects of MNTFs on neuronal survival and viability followingthe transection of the dorsal root ganglion in Sprague Dawley rats. Inthis series of experiments, axotomized dorsal root ganglion were cut inthe right side of 10 day-old Sprague Dawley rats. The left-side dorsalroot ganglion were left intact to serve as an internal contralateralcontrol. Sections of MNTF1-containing or MNTF2-containing PhastGel (˜2×3mm section containing 5-30 ng of MNTFs) were applied via implantation tothe axotomized sites. As before, the MNTF1 and MNTF2 correspond to the35 kD and 22 kD proteins, respectively. PhastGel sections without MNTFswere implanted in axotomized animals in an analogous manner, and servedas controls. Results demonstrated that without the presence of eitherMNTF1 or MNTF2, only ˜40% of the motoneurons remained viable (see FIG.12A). In contrast, 60-70% remained viable in the presence of MNTF1 (seeFIG. 12B), MNTF2 (see FIG. 1 2C) or both MNTF1 and MNTF2 (see FIG. 12D).

In Vivo Testing of MNTF1 and MNTF2 in Hemi-Sectioned Spinal Cords withNerve Autographs

[0067] In the next series of experiments, depicted in FIG. 13 and FIG.14 (panels 1-6), rat MNTF1 and MNTF2 were tested in vivo inhemi-sectioned spinal cord rats with peripheral nerve autographs.Following anesthesia with barbitol, the left side of the spinal cord ofadult Sprague Dawley rats were hemi-sectioned at the T1-T3 (8 mm) level.Under a dissection microscope, 20 mm long ulnar and median nerve trunksfrom the left fore limb were dissected and grafted onto the T1-T3 regionof the spinal cord at the lateral-ventral and lateral-dorsal portions,respectively (see FIG. 13). The nerve grafts were anchored to the spinalcord by suturing their associated membranes together with 10-0 thread.In the experimental group, a total of 16 sections (˜1×1 mm) ofMNTF1-containing and MNTF2-containing PhastGel were placed in closeproximity to the junctions of the nerve grafts and spinal cord. In acontrol group, PhastGel sections without MNTFs were implanted in ananalogous manner.

[0068] Results revealed that, in the presence of the MNTF1-containingand MNTF2-containing PhastGel sections (see FIG. 14, panels 1 and 3,respectively), the survival rate, the overall recovery rate, the numbersof both regenerated myelinated and unrnyelinated nerve fibers, and thenumber of surviving motoneurons of the hemi-sectioned rats (see FIG. 14,panel 3) were far greater than those exhibited by the control groupwithout the presence of MNTFs (see FIG. 14, panels 2 and 4). The resultswere obtained by both general and histological observations which wererecorded and compared. With respect to general observations, the rat'sambulatory ability (i.e., the ability to crawl or walk) and reaction todigit compression were recorded. Histological observations includeddetermination of inflammatory response (presence of infiltratingmacrophages, lymphocytes, and scar tissue), morphology andultrastructure of neurons, the presence of myelinating Schwann cells,and the presence of myelinated nerves. The results demonstrated that theexperimental animals treated with MNTFs recovered both motor and sensoryneuronal function in a far more efficacious manner than the controlanimal group. The experimental animal group also exhibited minimalinflammatory response, little or no scar tissue formation, normalSchwann cell morphology, and normal myelinated and umnyelinated nervemorphology (see FIG. 14, panel 3). In contrast, the control animal groupexhibited an inflammatory response, indicated by large numbers ofinfiltrating macrophages/lymphocytes and collagen-containing scar tissueformation at the location of the neuronal graft (see FIG. 14, panel 5).Furthermore, the Schwann cells of the control animal group were eithernon-viable or exhibited a swollen morphology with vacuolations (see FIG.14, panel 2).

[0069] In addition, subsequent experimental results demonstrated thatthe application of MNTF1 and/or MNTF2 significantly increased theamount, size, and neuronal shape of the regenerated myelinated nervefiber. From these results it may be postulated that MNTFs play a role inthe biosynthesis of the various protein constituents of myelin (e.g.,myelin basic proteins, Wolfgram proteins, etc.). Decreases in theconcentrations of these proteins is implicated in several degenerativediseases of myelinated nerve fibers.

In Vivo Testing of Rat MNTF1 and MNTF2 in Hereditary Motoneuron Disease

[0070] The molecular mechanism for human motoneuron diseases, includingAmotrophic Lateral Sclerosis (ALS) and Spinal Muscular Atrophies (SMA)have not yet been elucidated. However, the wobbler mouse (genotypewr/wr) has been developed and is widely used as an animal model forstudies involving spinal and brainstem motoneuron diseases. See e.g.,Duchen, L. W. and Strich, S. J., An Hereditary Motor Neuron Disease withProgressive Denervation of Muscle in the Mouse: The Mutant “Wobbler,” 31J. Neurol. Neurosurg. Psychiatry, 535 (1968); La Vail, J. H., et al.,Motoneuron Loss in the Abducens Nucleus of Wobbler Mice, 404 Brain Res.127 (1987), whose disclosures are incorporated herein by reference. Thewobbler mouse carries an inherited autosomal double-recessive genemutation in Chromosome 11 (specifically located between the egfr and relgenes), which leads to the progressive degeneration of spinal andbrainstem motoneurons. See Kaupman, K., et al., 13 Genomics 35 (1992),whose disclosure is incorporated herein by reference.

[0071] Approximately 3 week post-partum, wobbler mice begin to developthe “wobbling” symptomology (stage 1) with concomitant degeneration ofcervical motoneurons leading to both the wasting of the muscles of theforelimbs and an inability to extend the digits and claws. By 3 monthsof age, the pathologic symptomology will progress to stage 4, with a“clumping together” of all associated joints in the forelimbs (e.g., thewrist, elbow, and shoulder joints), as well as an extensive loss of bodyweight and chronic fatigue. Frequently, however, most wobbler mice dieprior to reaching 3 months of age.

[0072] The wobbler mouse model has been utilized in demonstrating thelimited protective effect of several trophic factors on the degenerationof motoneurons including: (i) the Ciliary Neurotrophic Factor (CNTF; seee.g., Sendner, M., et al., Ciliary Motoneurotrophic Factor Prevents theDegeneration of Motor Neurons After Axotomy, 345 Nature 440 (1990)),

[0073] (ii) the Brain-Derived Neurotrophic Factor (BDNF; see e.g.,Oppenheim, R. W., et al., Brain-Derived Motoneurotrophic Factor RescuesDeveloping Avian Motoneurons From Cell Death, 360 Nature 755 (1992)),(iii) the Ganglion-Derived Neurotrophic Factor (GDNF; see e.g.,Henderson, C. E., et al., GDNF: A Potent Survival Factor for MotoneuronsPresent in Peripheral Nerve and Muscle, 266 Science 1062 (1994), and(iv) the combination of CNTF and BDNF (see e.g., Mitsumoto, I. L., etal., 265 Science 1107 (1994)). In these aforementioned studies, thetreated wobbler mice were administered with milligram (mg)concentrations of the corresponding trophic factor at each dosing, witha treatment schedule consisting of several dosings per week for a periodof a number of weeks. In most cases, response by the wobbler mice totreatment with the trophic factor (as measured by improvement of motorfunction) were scored during a one month time period. Interestingly,however, the trophic factor-treated mice frequently died sooner than thecontrol wobbler mice which did not receive treatment. See e.g., Ikeda,S., et al., Histometic Effects of Cilliary Neurotrophic Factor inWobbler Mouse Motor Neuron Disease, 37 Ann. Neurol. 47 (1995); Ikeda,S., et al., Effects of Brain-Derived Neurotrophic Factor on MotorDysfunction in Wobbler Mouse Motor Neuron Disease, 37 Ann. Neurol. 505(1995).

[0074] The “negative” results demonstrated by these aforementionedtrophic factor studies raise the question of whether these investigatorswere working with the appropriate trophic factor(s) and/or theappropriate biological assays. Therefore, based upon the documentedrelationship between motoneurons and their associated target muscles,the inventor of the invention disclosed herein chose to isolate andpurify the motoneuronotrophic factors (MNTF1 and MNTF2) identified bythe motoneurons themselves from the target tissue of the respectivemotoneuron (i.e., the skeletal muscle); and to study the survival curveof the treated wobbler mice after only a single dose of MNTF1administered at nanogram (ng) concentrations for a period of one year.It should be noted that the MNTF concentration utilized in this studywas 1000-times less (i.e., ng versus mg concentrations) than thatutilized in previous studies involving several other motoneuronotrophicfactors (e.g., See e.g., Ikeda, S., et al., Histometic Effects ofCilliary Neurotrophic Factor in Wobbler Mouse Motor Neuron Disease, 37Ann. Neurol. 47 (1995); Ikeda, S., et al., Effects of Brain-DerivedNeurotrophic Factor on Motor Dysfunction in Wobbler Mouse Motor NeuronDisease, 37 Ann. Neurol. 505 (1995)).

[0075] In a series of experiments, depicted in FIGS. 15A and 15B, invivo testing of rat MNTF1 and MNTF2 in hereditary motoneuron disease wasperformed in wobbler mice. Wobbler mice are animals (developed at andkindly provided by the National Institutes of Health (NIH) in Bethesda,Md.) which possess double recessive genes (wr/wr) for a hereditarymotoneuron disease. The motoneuron disease manifests itself withsymptoms of upper limb neuromuscular failure which initially appearsapproximately 3 weeks after birth. The condition also affects theanimal's body weight, general health conditions, respiration, and lifespan in a deleterious manner. The disease gradually progresses to thefinal stage (stage 4) by 3 months of age, with an associated dramaticincrease in animal mortality.

[0076] Sections (˜2×6 mm) of MNTF1-containing and MNTF2-containingPhastGel were finely minced and applied to the wobbler mice viaimplantation of the aforementioned MNTF-containing PhastGel sectionsbetween the trapezius and rhomboid muscles at the C7-T3 region of thespinal cord. A control group of wobbler mice received PhastGel sectionswithout MNTFs. Results indicated that the application of a single “dose”(35 mg/kg body weight) of MNTF-containing PhastGel to 6 week old wobblermice arrested the progression of the symptomology of the motoneurondisease to that associated with the initial stage (stage 1) for a periodof observation up to 10 months. Additionally, in comparison to thecontrol animal group, the experimental animals demonstrated a generalimprovement in health, respiration, body weight, strength of fore limbs,as well as the general prevention of deterioration of health,respiration, neuromuscular activities of the fore limbs, and thevacuolation and chromatolysis of their cervical motoneurons. Inconclusion, these results are illustrative of rat MNTFs arresting thefurther symptomatic development of hereditary motoneuron disease inwobbler mice.

[0077]FIG. 15A and 15B graphically depicts the weights of homozygous andheterozygous wild type mice verses wobbler mice. In this series ofexperiments, the body weights of four “normal” homozygous mice (A) andfour heterozygous mice (B) were compared with the body weights of foureach homozygous wobbler mice (W1-W4) and (W5-W8). Each of the wobblermice were treated with rat MNTF1 and MNTF2, whereas the “normal” micewere left untreated. While, the overall body weights of the wobbler micewere generally less than that of “normal” animals, the wobbler micenonetheless exhibited consistent weight gain and increased longevity. Incontrast, untreated wobbler mice did not demonstrate this type ofconsistent weight gain and usually die at approximately 3 months of age.It should be noted that the abrupt “drop-points” in both FIG. 15A and15B represent the date on which that particular animal expired.Therefore, in conclusion, these results indicate that treatment with theMNTFs had a dramatic impact on the hereditary motoneuron disease inwobbler mice.

[0078] The elucidation of the relationship between the individual musclefibers and their associated motomeurons is of paramount importance inunderstanding such diseases as multiple sclerosis (MS), musculardystrophy (MD), and myasthenia gravis (MG). In a related series ofexperiments, it was demonstrated that the application of MNTF1 and/orMNTF2 which resulted in a marked regeneration of the motorneuronsconcomitantly led to the recovery associated muscle fibers formation.Moreover, neuro-stimulation of the muscle fiber to promote the growth,survival, and regeneration of the muscle fiber, as well as for theputative production of muscle-derived MNTFs, plays an extremelyimportant role in the cyclic relationship between motorneurons and themuscle fibers.

Development of Monoclonal Antibodies for MNTF1 and MNTF2

[0079] Monoclonal antibodies specific for rat MNTF1 and MNTF2 wereprepared. The 35 kD (MNTF1) and 22 kD (MNTF2) protein-containing bandswere excised from a Phast System gel (1×30 mm gel sections containing˜100 ng motoneuronotrophic factor) and utilized as antigens in theimmunization of separate groups of Balb/c mice. Specifically, theMNTF-containing PhastGel sections were excised and finely minced. ThePhastGel pieces were then mixed with an equal volume of completeFreund's adjuvant and directly injected intraperitoneally into theBalb/c mice. A total of three antigen immunizations were performed, withintraperitoneal injections of MNTF1-containing and MNTF2-containingPhastGel in physiological saline on the 7th and 21st day following theinitial immunization. The spleens of the Balb/c mice were harvested andallowed to fuse with either NS-1 or SP2/0 myeloma cells. The resultanthybridomas were then screened utilizing MTT microassays and microscopicexamination to select the most efficacious monoclonal antibodies capableof blocking the function of the MNTF1 and MNTF2 neuronotrophic factors.For example, from the 35 kD (MNTF1) fusions, a total of 8 hybridomaswere selected and subsequently developed out of a total of 480 wellsproducing monoclonal antibodies specific for this aforementioned MNTF1protein.

Immunoselection of Recombinant Human MNTF1

[0080] The selected MNTF-blocking monoclonal antibodies were nextutilized to immunoselect clones of human motoneuronotrophic factor. Theblocking monoclonal antibody for the human motoneuronotrophic factor(MAb-MNTF) was used as an immunoprobe in the screening of positiveexpression clones from a selected human retinoblastoma cDNA library(produced by Clontech Co., Palo Alto, Calif.). In brief, theimmunoselection/immunoprecipitation procedure for the cDNA libraryinvolved the isolation of mRNAs from cells of the Rb Y-79 retinoblastomacell line. Reverse transcription reaction were performed to synthesizecDNAs utilizing the isolated retinoblastoma mRNAs as templates. ThecDNAs were ligated into a Lambda phage vector (gt-11) and subsequentlytransformed in an E. coli strain Y1090 host bacteria.

[0081] The immunoscreening procedure utilized was a modification of thatdescribed in Young, R. A., and Davis, R. W., Efficient Isolation ofGenes Using Antibody Probes, 80 Proc. Nat'l Acad. Sci. USA 1194 (1983),whose disclosure is incorporated herein by reference. The modificationconsisted of using ammonium nickel sulfate as an enhancing agent toincrease 100-fold the sensitivity of the peroxidase-avidin-biotincomplex reaction utilizing diamino-benzidine as the substrate. A maximumof 4 nitrocellulose membrane “replicas” were made from each colony platefor immunoscreening and only the most intensely-stained clones wereselected in the immunoscreening procedure. The selected clones were thenallowed to express their recombinant human proteins which weresubsequently assayed to determine their potential MNTF biologicalactivity in culture. The clone which displayed the highest MNTF-specificbiological activity in motoneuron cultures was then selected for furtheranalysis.

Immunohistochemical Localization of MNTF1 and the MNTF1 Receptor

[0082] The MNTF1 monoclonal antibody was utilized to elucidate theimmunohistochemical distribution of MNTF1 and the putative MNTF1receptor in the peroneal muscle, Schwann cells, and motoneurons of 10day-old Sprague Dawley rats. The procedure utilized was previouslydescribed in Ren, F. & Chau, R. M. W., Production and Assessment ofMonoclonal Antibodies Specific for Rat Retinal Ganglion NeuronotrophicFactor, 7 J. Monoclonal Antibody 13 (1991). FIG. 5 demonstrates theresults of this immunohistochemical localization of the MNTF1 protein(plates 1, 3 and 5) in peroneal muscle (plates 1 and 2), Schwann cells(plates 3 and 4) and motoneurons (plates 5 and 6). The results show anuneven distribution of fine, immunoreactive-positive “staining” amongthe muscle fibers with the more dense staining localized at theT-tubules, the motor end-plates, and the intervening nerve fibers. Thestriations of the individual muscle fibers were clearly visible withoutthe utilization of a counter-stain. In addition FIG. 5 illustrates theimmunohistochemical localization of the putative MNTF1 receptor (plates2, 4, and 6).

Cloning and Sequence Analysis of Human Recombinant MNTF1

[0083] Human recombinant MNTF1 (hrMNTF1) was tested in vitro forpotential biological activity with isolated anterior horn motoneurons inthe following manner (see FIG. 16, panels A-F). Anterior hornmotoneurons were isolated from the lumbar spinal cord gray matter of 10day-old Sprague Dawley rats following digestion in a 0.08% collagenasesolution in DMEM medium containing 15% fetal calf serum. Followingcollagenase digestion, the anterior horn motoneurons were cultured inDMEM medium supplemented with 15% fetal calf serum. Results indicatedthat the anterior horn motoneurons co-cultured with hrMNTF1 remainedviable and exhibited higher levels of neurite outgrowth (see FIG. 16,panel E and panel F) than that of the control cultures without hrMNTF1(see FIG. 16, panel D). Viability and neurite outgrowth were determinedutilizing both MTT microassay and microscopic examination as previouslydescribed.

[0084] The cloned, recombinant human MNTF1 in gt-11 phage vector(designated as Lambda.35KD.MNTF1) was expressed in E. coli strain Y1090.It was found, in vitro, to be capable of reducing, by a factor of3-fold, the overall numbers of isolated anterior horn motor neuronswhich entered into apoptosis (the process of cellular “dying”) asevidenced by a lack of cellular fragmentation into apoptotic bodies andthe condensation of chromatin in the pyknotic nucleus. In addition,hrMNTF1 supported the growth and “spreading” of the motoneurons intogiant, active neurons with extended growth cone-containing axons (seeFIG. 16, panels E and panel F). In contrast, the control motoneuroncultures, lacking expressed hrMNTF1, many non-neuronal cells (e.g.,glial cells and fibroblasts) were actively growing after 10 days ofculture in DMEM medium supplemented with 15% fetal calf serum (see FIG.16, panel D), yet there was a complete lack of viable, growingmotoneurons in these control cultures.

[0085] The cDNA insert of the Lambda.35KD.MNTF1 clone was thensub-cloned into an in vitro expression vector system and its DNAsequence was elucidated by the following methodologies:

1. Preparation and Purification of Lambda.35KD.MNTF1

[0086] 10 plaque-forming units (pfu) of Lambda.35KD.MNTFl/gt 11 phagewas inoculated into a 500 ml overnight culture of E. coli stain Y1090until complete lysis of the bacteria was observed. The Lambda phage wererecovered and purified by centrifugation and enzymatic treatment withRNase and DNase in an NaCl/PEG 8000 solution, followed by high speedCsCl density gradient centrifugation to collect the purified phage at afinal gradient density of approximately 1.5 gm CsCl/ml.

[0087] Isolation and purification of the Lambda.35KD.MNTF1 DNA wasfacilitated by initial digestion of the Lambda phage (1 ml) in EDTA,SDS, and proteinase K, followed by repeated extractions with phenol,phenol/chloroform, and chloroform. The DNA was then ethanol precipitatedwith 95% ethanol and collected by centrifugation. Following repeatedwashes in 70% ethanol, the Lambda.35KD.MNTF1 DNA pellet was redissolvedin Tris-EDTA buffer.

2. Recovery of 35KD.MNTF1 cDNA from Lambda.35KD.MNTF1 DNA

[0088] 10 μg of Lambda.35KD.MNTF1 DNA was digested overnight with EcoR1and the cleaved 35KD.MNTF1 cDNA was recovered via agarose gelelectrophoresis. Following electrophoresis, the DNA bands werevisualized utilizing U.V. light. Results of the EcoR1 digestiondemonstrated the presence of two discreet DNA fragments—a 1.44 Kbpfragment designated 35F3 and a 0.93 Kbp fragment designated 35F6. Thetwo DNA bands were individually excised from the agarose gel and the DNAwas recovered via standard techniques. The recovered DNA was thenprepared for subsequent DNA sequencing by recombination with M13 phageand for High Protein Expression by recombination with the pGEX-1 LambdaT EcoR1/BAP vector.

3. DNA Sequencing of the 35F3 and 35F6 DNA Fragments of 35KD.MNTF1 cDNA

[0089] Following recombination of the 35F3 and 35F6 fragments with M13,the Dideoxy Nucleotide Chain Termination DNA Sequencing Methodology ofSanger (utilizing Bst DNA polymerase) was employed to elucidate the DNAsequence of the two MNTF1 DNA fragments. See Sanger, F., et al.,Nucleotide Sequence of Bacteriophage DNA, 162 J. Mol. Biol. 729 (1982),whose disclosure is incorporated herein by reference. This sequencingmethodology provided extremely accurate and reproducible results withrespect to the DNA sequencing of the two EcoR1-generated fragments ofthe 35KD.MNTF1 CDNA fragments and indicated that the 35F3 fragmentconsisted of 1443 base pairs; whereas the 35F6 fragment was found toconsist of 927 base pairs.

[0090]FIG. 1A depicts the DNA sequence (SEQ ID NO:1) of the 35F3 DNAfragment (1443 bp). By standard convention the DNA sequence is shown inthe 5′ to 3′ orientation. The 35F3 clone was constructed by EcoR1digestion of the Lambda 35KD.MNTF1 clone. The resulting 35F3EcoR1-generated fragment was then recombined with M13 for subsequent DNAsequencing utilizing the aforementioned Sanger methodology.

[0091]FIG. 1B depicts the DNA sequence (SEQ ID NO:2) of the 35F6 DNAfragment (927 bp). By standard convention the DNA sequence is shown inthe 5′ to 3′ orientation. The 35F6 clone was constructed by EcoR1digestion of the Lambda 35KD.MNTF1 clone. The resulting 35F6EcoR1-generated fragment was then recombined with M13 for subsequent DNAsequencing utilizing the aforementioned Sanger methodology.

4. Sub-Cloning of the 35F3 and 35F6 EcoR1-Generated MNTF1 DNA Fragments

[0092] The 35F3 (1443 base pairs) and 35F6 (927 base pairs)EcoRl-generated MNTF1 DNA fragments were sub-cloned into the pGEX-1Lambda T EcoR1/BAP High Protein Expression vector (hereinafter pGEX).The resultant sub-clones were designated MNTF1-1443 (35F3) and MNTF1-927(35F6). The pGEX vector was selected due to the following factors: (i)it possessed a high efficiency transcriptional promotor at its5′-terminus and a 3′-terminus poly(A) tail; and (ii) it provided an easymethodology for the purification of the MNTF1-1443 and MNTF1-927recombinant proteins via affinity column chromatography-basedpurification of the glutathione-S-transferase (GST)-containing MNTF1fusion proteins.

A. Transformation of Recombinant Plasmid

[0093] The pGEX vector was digested with EcoR1. The digested pGEX vectorwas then incubated overnight with the EcoR1-generated MNTF1-1443 andMNTF1-927 DNA fragments in the presence of T4 DNA ligase. Followingligation, competent E. coli strain DH5 were transformed with therecombinant vector and transferred onto Luria broth (LB) agar platescontaining 100 g/ml ampicillin. Due to the fact that the pGEX vectorcontained a gene which conferred ampicillin resistance to thetransformed bacteria, the use of ampicillin screening allowed theexclusive selection of transformed bacterial colonies, as only thosebacteria containing the recombinant vector would be viable in itspresence.

B. Identification and Isolation of MNTF1-1443 and MNTF1-927Transformants

[0094] The transformed bacterial colonies were individually selected,inoculated into a small volume of LB medium containing 100 g/mlampicillin, and incubated for approximately 3 hours. Followingincubation, the transformed bacteria were collected via centrifugationfor subsequent isolation of the recombinant vector DNA by the alkalinelysis “mini prep” technique as described in Maniatis, T., Fritsch, E.F., and Sambrook, J., Molecular Cloning, 2nd ed., Cold Spring HarborLaboratory, Cold Spring Harbor, New York pp. 134-136 (1986). With thistechnique, the collected bacteria were lysed by the addition of aTris-EDTA/NaOH/SDS solution with vigorous vortexing. Aftercentrifugation to collect contaminating cellular debris, the recombinantvector DNA-containing supernatant was aspirated, extracted with phenoland chloroform, and precipitated with 95% ethanol. The mixture wascentrifuged to collect the precipitated DNA and the nucleic acid pelletwas dissolved in Tris-EDTA buffer. Two recombinant species wereidentified: pGEX-MNTF1-1443 and pGEX-MNTF1-927.

[0095] The collected recombinant vector DNA was then digested with EcoR1to release the 1443 bp MNTF1-1443 and 927 bp MNTF1-927 DNA inserts fromthe 4.9 Kbp pGEX vector. The digested DNA was subjected to agarose gelelectrophoresis and the individual DNA bands were identified via U.V.light visualization.

[0096] “Positive” bacterial colonies (i.e., those which contained eitherthe MNTF1-1443 or MNTF1-927 DNA insert) were selected and inoculatedinto LB medium for large scale plasmid purification via alkaline lysisand PEG precipitation. The purified recombinant vector DNA was thentransfected into an E. coli strain BL-21 host bacterium to facilitatehigh levels of expression of human recombinant MNTF1-F3 (hrMNTF1-F3) andMNTF1-F6 (hrMNTF1-F6) proteins.

C. Expression and Amplification of hrMNTF1-F3 and hrMNTF1-F6 FusionProteins

[0097] pGEX-MNTF1-1443 or pGEX-MNTF1-927 DNA were transfected into E.coli stain

[0098] BL-21 competent bacteria in 50 ml of LB medium. IPTG was utilizedto induce high levels of expression of the hrMNTF1-F3 or hrMNTF1-F6proteins. Collected bacteria were lysed, frozen, and thawed a total of4-times using liquid nitrogen, sonicated, and centrifuged. Thesupernatant, containing the hrMNTF1-F3 or hrMNTF1-F6 fusion proteins,was then passed through an anti-GST affinity chromatography columncontaining anti-GST monoclonal antibodies (Pharmacia). The use of thistype of affinity chromatography allowed purification of theGST-hrMNTF1-F3 and GST-hrMNTF1-F6 fusion proteins which were bound tothe matrix through the GST moiety. Following a high salt wash, theGST-hrMNTF1-F3 and GST-hrMNTF1-F6 fusion proteins were eluted. Theproteolytic enzyme thrombin was utilized to cleave the linkage betweenthe MNTFs and the GST moiety and the purified hrMNTF1-F3 and hrMNTF1-F6proteins were collected for subsequent in vivo and in vitro assays andfor amino acid sequencing.

In Vitro and In Vivo Testing of the Trophic Activity of hrMNTF1-F3 andhrMNTF1-F6

[0099] The human recombinant motoneuronotrophic factors (hrMNTFs) may beutilized either as fusion proteins or as purified proteins. To obtainthe purified protein form of hrMNTF1-F3 and MNTF1-F6, the expressionproducts of the pGEX-MNTF1-1443 or pGEX-MNTF1-927 plasmids are isolatedas previously described. The expression products consist of fusionproteins which contain MNTF fused with glutathione-S-transferase (GST).The fusion protein is then purified on a GST-monoclonal antibodyaffinity chromatography column (Pharmacia) which specifically cleave thebond between the expressed trophic factor (MNTFs) and the GST moiety.The trophic factor is subsequently eluted from the column and may beutilized in the same manner as the purified “native” proteins, asdescribed above.

[0100] Anterior horn motoneurons were isolated from the 10 day-old(postnatal) Sprague Dawley rat lumbar spinal cord via collagenasedigestion in DMEM medium supplement with 15% fetal calf serum. FIG. 17represents low magnification (100×) photomicrographs of theaforementioned anterior horn motoneurons cultured with varyingconcentrations of the GST-hrMNTF1-F3 (panel 4), GST-hrMNTF1-F6 (panel3), and hrMNTF1-F3 (panel 6) and hrMNTF-F6 (panel 5) proteins with theGST moiety removed by thrombin proteolysis were added to the motoneuroncultures. Following 8 days of culture, it was observed that themotoneurons cultured in the presence of GST-conjugated ornon-GST-conjugated hrMNTF1-F3 or hrMNTF1-F6 exhibited far greater growthpatterns, as well as a marked decrease in the growth of associatednon-neuronal cells, than those motoneurons cultured without either ofthese aforementioned proteins (see FIG. 17, panel 1 and 2). Thesimilarity of the results obtained between motoneuron cultures withhrMNTF1-F3 and hrMNTF1-F6 suggested that both pGEX-MNTF1-1443 andpGEX-MNTF1-927 may potentially possess the gene, or part of the genewhich encodes the active site of a biologically active neurotropicfactor.

[0101]FIG. 18 depicts high magnification (320x) photomicrographs of theresults initially illustrated in FIG. 17. It should be noted that panels1-6 in FIG. 18 are identical to those panels shown in FIG. 17 withrespect to the type of MNTF protein which was utilized in the co-culturewith the isolated anterior horn motoneurons.

[0102]FIG. 19 represents a high magnification (400×) photomicrograph ofmotoneuron viability and neurite outgrowth of in vitro-isolated anteriorhorn motoneurons cultured with hrMNTF1-F6 which has had the GST moietyremoved by thrombin proteolysis.

[0103] FIGS. 20A-D depict the survival, regeneration and neuriteoutgrowth of myelinated nerve fiber from the in vitro-isolated anteriorhorn motoneurons as originally shown in FIG. 17 (plate 5), FIG. 18(plate 5), and FIG. 19 (plate 1), respectively, after 21 days ofco-culture with the hrMNTF1-F6 protein with the GST moiety removed viathrombin proteolysis. FIG. 20A illustrates a low magnificationphotomicrograph (100×) of two separate motoneurons (one ˜1.5 mm inlength and one ˜3 mm in length) which were regenerated from thecultured, in vitro-isolated anterior horn motoneurons. FIG. 20B depictsa low magnification (140×) photomicrograph of the motoneurons originallyillustrated in FIG. 20A as well as a composite photomicrograph whichillustrates the myelinated, regenerated neuron with oligodendrocytes(putative Schwann cells) attached. FIGS. 20C and 20D illustrate highmagnification (400×) photomicrographs which revel the axonal cylindersin the inner regenerated myelinated nerve fiber with several Nodes ofRannier clearly depicted.

Amino Acid Sequencing of the Human Recombinant MNTF1-F3 and MNTF1-F6Proteins

[0104] The amino acid sequence of the human recombinant MNTF1-F3 andMNTF1-F6 proteins were elucidated by direct protein sequencingmethodologies. Prior to sequencing, the proteins were purified intonon-fusion form via the aforementioned GST-monoclonal affinity columnchromatography. The amino acid sequence of the MNTF1-F3 protein (SEQ IDNO:3) is shown in FIG. 2A. The amino acid sequence of the MNTF1-F6protein (SEQ ID NO:4) is shown in FIG. 2B. By standard convention, theamino acid sequences are reported from the amino (NH2—) terminus to thecarboxyl (—COOH) terminus.

[0105] The MNTF polypeptides described herein can be used for treatmentof numerous medical conditions including, for example, peripheral nerveinjuries, musculoskeletal disorders, spinal cord injuries, headinjuries, strokes, neuromuscular degenerative diseases, amyotrophiclateral sclerosis, spinal muscular atrophy, peripheral neuropathy,inhibition of scar tissue formation, diabetic peripheral neuropathy;peripheral neuropathy resulting from AIDS, peripheral neuropathyresulting from radiation treatment for cancer, multiple sclerosis,muscular dystrophy, myasthenia gravis, and sensory neuronal functiondisorders.

Preparation of MNTF for Drug Delivery

[0106] The MNTFs of the present invention can thus be readily utilizedin pharmacological applications. In vivo applications includeadministration of the factors to mammalian subjects and, in particular,to human subjects. The MNTFs may be administered orally or by injection,with the preferred mode being topical application on or near theaffected motoneuron.

[0107] The pharmacological compositions of the present invention areprepared in conventional dosage unit forms by the incorporation of oneor more of the MNTFs with an inert, non-toxic pharmaceutical “carrier”moiety according to accepted methodologies, in a non-toxic concentrationsufficient to produce the desired physiological activity in a mammaland, in particular, a human subject. Preferably, the compositioncontains the active ingredient in a biologically-active, but non-toxic,concentration selected from a concentration of approximately 5 ng to 50mg of active ingredient per dosage unit (e.g., per kg subject bodyweight). The concentration utilized will be dependent upon such factorsas the overall specific biological activity of the ingredient, specificbiological activity desired, as well as the condition and body weight ofthe subject.

[0108] The pharmaceutical carrier or vehicle employed may be, forexample, a solid or liquid and a variety of pharmaceutical forms may beemployed. Thus, when a solid carrier is utilized, the preparation may beplain milled, micronized in oil, tabulated, placed in a hard gelatin orenterically-coated capsule in micronized powder or pellet form, or inthe form of a troche, lozenge, or suppository. The solid carrier,containing the MNTF, can also be ground up prior to use.

[0109] When utilized in a liquid carrier, the preparation may be in theform of a liquid, such as an ampule, or as an aqueous or non-aqueousliquid suspension. For topical administration, the active ingredient maybe formulated using bland, moisturizing bases, such as ointments orcreams. Examples of suitable ointment bases include, but are not limitedto, petrolatum plus volatile silicones, lanolin, and water in oilemulsions such as Eucerin® (Beiersdorf). Examples of suitable creambases include, but are limited to, Nivea Cream® (Beiersdorf), cold cream(USP), Purpose Cream® (Johnson & Johnson), hydrophilic ointment (USP),and Lubriderm® (Warner-Lambert).

[0110] Additionally, with respect to the present invention, the activeingredient may be applied internally at or near the site of the affectedmotoneuron. For example, a solid or gelled medium which is sufficientlypermeable to allow the release of the active ingredient, preferably in atimed-release manner, may be utilized for such internal application.Examples of such gels include, but are not limited to, hydrogels such aspolyacrylamide, agarose, gelatin, alginate, or other polysaccharidegums. Furthermore, the active ingredient may be imbedded in a solidmaterial, such as filter paper, which is capable of absorbing andsubsequently releasing the active ingredient, at the appropriate timeand location.

[0111] While embodiments and applications of the present invention havebeen described in some detail by way of illustration and example forpurposes of clarity and understanding, it would be apparent to thoseindividuals whom are skilled within the relevant art that manyadditional modifications would be possible without departing from theinventive concepts contained herein. The invention, therefore, is not tobe restricted in any manner except in the spirit of the appended claims.

1 5 1 1443 DNA Homo Sapiens 1 cgggcttatt attccactga tgagaacctgatcctttccc cactcctggg taacgtctgc 60 ttctccagct cccagtacag catctgcttcacgctgggct cctttgccaa gatctatgcc 120 gacacctttg gtgacattaa ttaccaagaatttgctaaaa gactctgggg tgacatctac 180 ttcaacccta agacgcgaaa gttcaccaaaaaggccccaa ctagcagctc ccagagaagt 240 ttcgtggagt ttatcttgga gcctctttataagatcctcg cccaggttgt aggtgacgtg 300 gacaccagcc tcccacggac cctagacgagcttggcatcc acctgacgaa ggaggagctg 360 aagctgaaca tccgcccctt gctcaggctggtctgcaaaa agttctttgg cgagttcaca 420 ggctttgtgg acatgtgtgt gcagcatatcccttctccaa aggtgggcgc caagcccaag 480 attgagcaca cctacaccgg tggtgtggactccgacctcg gcgaagctat gagtgactgt 540 gaccctgatg gccccctgat gtgccacactactaagatgt tcagcacaca tgatggagtc 600 cagtttcacc cctttggccg ggtgctgagtggcaccattc atgctgggca gcctgtgaag 660 gttctggggg agaactacac cctggaggatgaggaagact ccccaatttg ccccgtgggc 720 cgcctttgga tctctgtggc cagctaccacatcgaggtga accgtgttcc tgctggcaac 780 tgggttctga ttgaaggtgt tgatcaaccaattgtgaaga cagcaaccat aaccgaaccc 840 cgaggcaatg aggaggctca gattttccgacccttgaagt tcaataccac atctgttatc 900 aagattgctg tggagccagt caacccctcagagctgccca agatgcttga tggcctgcgc 960 aaggtcaaca agagctatcc atccctcaccaccaaggtgg aggagtctgg cgagcatgtg 1020 atcctgggca ctggggagct ctacctggactgtgtgatgc atgatttgcg gaagatgtac 1080 tcagagatag acatcaaggt ggctgacccagttgtcacgt tttgtgagac ggtcgtggaa 1140 acatcctccc tcaagtgctt tgctgaaacgcctaataaga agaacaagat caccatgatt 1200 gctgagcctc ttgagaaggg cctggcagaggacatagaga atgaggtggt ccagattacg 1260 tggaacagga agaagctggg agagttcttccagaccaagt acgattggga tctgctggct 1320 gcccgttcca tctgggcttt tggccctgatgcgactggcc ccaacattct ggtggatgat 1380 actctgccct ctgaggtgga caaggctcttcttggttcag tgaaggacag catcgttcaa 1440 ggt 1443 2 927 DNA Homo Sapiens 2ttggggacat tttggggtga cacactgaac tgctggatgc tatcagcatt tagtaggtat 60gctcgatgtc ttgcagaagg acatgatggt cctacacagt aaggaatgga ttacctacaa 120tattaatagc agcctcccat acacactttt gacacccttc cctaaaggat taatatgctc 180caaccttcct gtccccacag ttcagtggct ctccctaccc tcaccatgat cggatgaaaa 240aaaataaggt ttcacagctt aagagtgaaa ttctggaatc caactacaag ctcataactg 300tagcatggaa cctggtagta gcataataaa taaattttta gtaagaggct taagaaattt 360tagcaaaaaa agcactccct ttcttcctcc ctacatatct catatgtttt tcaacacaaa 420aaattctgtg attttagaga aacttcttac agtactttta agttcaaaac cagatgctca 480ttacagttct tttaaacacc aaactagtca tctcaaaaat atggctaact ctctggacta 540aattccatag gaaaaattat taatttcaaa atgcctaatt tttgatcaat gctgaagagc 600caagcaatca tgtcctgctt ctcactcagg gcagagttct caggtcagaa gctccggagt 660ctgtcagaga ttaaaatatc atctcaacaa ttcacaagct acttctaagt gttaccctaa 720attagtcact aatcgtttct cccccaactc tatttcacaa attaaagttt acagaattga 780caaaaaccaa accaatgaaa caacccaggc tatttgcagg gggggggaaa gagatacccc 840aaaagtcaac cctatttaca cgtagttaaa agagtgatcc aacagatatt accctccata 900aagtacctaa aggcaggagc cggaatt 927 3 481 PRT Homo Sapiens 3 Arg Ala TyrTyr Ser Thr Asp Glu Asn Leu Ile Leu Ser Pro Leu Leu 1 5 10 15 Gly AsnVal Cys Phe Ser Ser Ser Gln Tyr Ser Ile Cys Phe Thr Leu 20 25 30 Gly SerPhe Ala Lys Ile Tyr Ala Asp Thr Phe Gly Asp Ile Asn Tyr 35 40 45 Gln GluPhe Ala Lys Arg Leu Trp Gly Asp Ile Tyr Phe Asn Pro Lys 50 55 60 Thr ArgLys Phe Thr Lys Lys Ala Pro Thr Ser Ser Ser Gln Arg Ser 65 70 75 80 PheVal Glu Phe Ile Leu Glu Pro Leu Tyr Lys Ile Leu Ala Gln Val 85 90 95 ValGly Asp Val Asp Thr Ser Leu Pro Arg Thr Leu Asp Glu Leu Gly 100 105 110Ile His Leu Thr Lys Glu Glu Leu Lys Leu Asn Ile Arg Pro Leu Leu 115 120125 Arg Leu Val Cys Lys Lys Phe Phe Gly Glu Phe Thr Gly Phe Val Asp 130135 140 Met Cys Val Gln His Ile Pro Ser Pro Lys Val Gly Ala Lys Pro Lys145 150 155 160 Ile Glu His Thr Tyr Thr Gly Gly Val Asp Ser Asp Leu GlyGlu Ala 165 170 175 Met Ser Asp Cys Asp Pro Asp Gly Pro Leu Met Cys HisThr Thr Lys 180 185 190 Met Phe Ser Thr His Asp Gly Val Gln Phe His ProPhe Gly Arg Val 195 200 205 Leu Ser Gly Thr Ile His Ala Gly Gln Pro ValLys Val Leu Gly Glu 210 215 220 Asn Tyr Thr Leu Glu Asp Glu Glu Asp SerGln Ile Cys Thr Val Gly 225 230 235 240 Arg Leu Trp Ile Ser Val Ala ArgTyr His Ile Glu Val Asn Arg Val 245 250 255 Pro Ala Gly Asn Trp Val LeuIle Glu Gly Val Asp Gln Pro Ile Val 260 265 270 Lys Thr Ala Thr Ile ThrGlu Pro Arg Gly Asn Glu Glu Ala Gln Ile 275 280 285 Phe Arg Pro Leu LysPhe Asn Thr Thr Ser Val Ile Lys Ile Ala Val 290 295 300 Glu Pro Val AsnPro Ser Glu Leu Pro Lys Met Leu Asp Gly Leu Arg 305 310 315 320 Lys ValAsn Lys Ser Tyr Pro Ser Leu Thr Thr Lys Val Glu Glu Ser 325 330 335 GlyGlu His Val Ile Leu Gly Thr Gly Glu Leu Tyr Leu Asp Cys Val 340 345 350Met His Asp Leu Arg Lys Met Tyr Ser Glu Ile Asp Ile Lys Val Ala 355 360365 Asp Pro Val Val Thr Phe Cys Glu Thr Val Val Glu Thr Ser Ser Leu 370375 380 Lys Cys Phe Ala Glu Thr Pro Asn Lys Lys Asn Lys Ile Thr Met Ile385 390 395 400 Ala Glu Pro Leu Glu Lys Gly Leu Ala Glu Asp Ile Glu AsnGlu Val 405 410 415 Val Gln Ile Thr Trp Asn Arg Lys Lys Leu Gly Glu PhePhe Gln Thr 420 425 430 Lys Tyr Asp Trp Asp Leu Leu Ala Ala Arg Ser IleTrp Ala Phe Gly 435 440 445 Pro Asp Ala Thr Gly Pro Asn Ile Leu Val AspAsp Thr Leu Pro Ser 450 455 460 Glu Val Asp Lys Ala Leu Leu Gly Ser ValLys Asp Ser Ile Val Gln 465 470 475 480 Gly 4 33 PRT Homo Sapiens 4 LeuGly Thr Phe Trp Gly Asp Thr Leu Asn Cys Trp Met Leu Ser Ala 1 5 10 15Phe Ser Arg Tyr Ala Arg Cys Leu Ala Glu Gly His Asp Gly Pro Thr 20 25 30Gln 5 99 DNA Homo Sapiens 5 ttggggacat tttggggtga cacactgaac tgctggatgctatcagcatt tagtaggtat 60 gctcgatgtc ttgcagaagg acatgatggt cctacacag 99

What is claimed is:
 1. A method for promoting the survival, growth,proliferation, or maintenance of mammalian neurons comprisingadministering to the neurons an effective amount of a purifiedpolypeptide comprising an amino acid sequence that is 100% identical toSEQ ID NO:4 wherein the neurons are spinal cord neurons in a severedspinal cord.
 2. The method of claim 1, wherein the polypeptide is atleast 95% identical to SEQ ID NO:4.
 3. The method of claim 1, whereinthe polypeptide is at least 90% identical to SEQ ID NO:4.
 4. The methodof claim 1, wherein the polypeptide is at least 85% identical to SEQ IDNO:4.
 5. The method of claim 1, wherein the polypeptide is 80% identicalto SEQ ID NO:4.
 6. The method of claim 1, wherein the method comprisespromoting regeneration of the axon of a spinal cord neuron in a severedspinal cord.
 7. The method of claim 1, wherein the method comprisespromoting the survival, growth, proliferation, or maintenance of neuronsin vitro.
 8. The method of claim 1, wherein the method comprisespromoting the survival, growth, proliferation, or maintenance ofisolated spinal motoneurons in vitro.
 9. The method of claim 1, whereinthe method comprises administering the polypeptide to non-neuronal cellsor tissues sufficiently proximal to spinal cord neuron in a severedspinal cord such that the polypeptide is effective at promoting thesurvival, growth, proliferation, or maintenance of spinal cord neuron ina severed spinal cord.
 10. The method of claim 1, wherein the methodcomprises promoting the rescue and morphologically-complete neuronalregeneration of axotomized spinal cord neuron in a severed spinal cordin vivo.
 11. The method of claim 1, wherein the method comprisespromoting the regeneration of nerve fibers in a severed spinal cord of amammal.
 12. The method of claim 1, wherein the method comprisespromoting the axonal regeneration of axotomized spinal cord neuron in asevered spinal cord in a mammal.
 13. The method of claim 1, wherein themethod comprises administering the polypeptide in a suitable carrier toa mammal for treatment of a medical condition of severed spinal cord.The method of claim 1 wherein the sequence of which comprises SEQ IDNO:4 with one or more conservative amino acid substitution
 14. A methodfor promoting the survival, growth, proliferation, or maintenance ofmammalian neurons comprising administering to the neurons an effectiveamount of a purified polypeptide comprising an amino acid sequence thatis at least 100% identical to SEQ ID NO:3 wherein the neurons are spinalcord neuron in a severed spinal cord.
 15. The method of claim 14,wherein the polypeptide is at least 95% identical to SEQ ID NO:3. 16.The method of claim 14, wherein the polypeptide is at least 90%identical to SEQ ID NO:3.
 17. The method of claim 14, wherein thepolypeptide is at least 85% identical to SEQ ID NO:3.
 18. The method ofclaim 14, wherein the polypeptide is 80% identical to SEQ ID NO:3. 19.The method of claim 14, wherein the method comprises promotingregeneration of the axon of a spinal cord neuron in a severed spinalcord.
 20. The method of claim 14, wherein the method comprises promotingthe survival, growth, proliferation, or maintenance of spinal cordneuron in a severed spinal cord in vitro.
 21. The method of claim 14,wherein the method comprises promoting the survival and growth ofisolated spinal motoneurons in vitro.
 22. The method of claim 14,wherein the method comprises promoting the rescue andmorphologically-complete neuronal regeneration of axotomized spinal cordneuron in a severed spinal cord in vivo.
 23. The method of claim 14,wherein the method comprises promoting the regeneration of nerve fibersin a severed spinal cord of a mammal.
 24. The method of claim 14,wherein the method comprises promoting the axonal regeneration ofaxotomized spinal cord neuron in a severed spinal cord in a mammal. 25.The method of claim 14, wherein the method comprises administering thepolypeptide to non-neuronal cells or tissues sufficiently proximal tospinal cord neuron in a severed spinal cord such that the polypeptide iseffective at promoting the survival, growth, proliferation, ormaintenance of mammalian spinal cord neuron in a severed spinal cord.26. The method of claim 14, wherein the method comprises administeringthe polypeptide in a suitable carrier to a mammal for treatment of amedical condition of severed spinal cord.
 27. The method of claim 14 forpromoting the survival, growth, proliferation, or maintenance ofmammalian spinal cord neuron in a severed spinal cord comprisingadministering to the spinal cord neuron in a severed spinal cord aneffective amount of a purified polypeptide, the sequence of whichcomprises SEQ ID NO:3 with one or more conservative amino acidsubstitution.